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bacterial expression vector encoding gfp nanobody  (Addgene inc)


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    Addgene inc bacterial expression vector encoding gfp nanobody
    Drift correction and localization error of QD probes in fixed hippocampal neurons expressing the YFP-tagged GluN1-1a subunit. A , Representative images of hippocampal neurons. Left, Neurons were stained by incubation in 20 n m MitoTracker Deep Red FM marker (catalog #M22426, Thermo Fisher Scientific) for 30 s in Neurobasal medium. Right, Neurons were infected with a lentivirus expressing the synaptic protein tdTomato-Homer1c. B , Schematic diagram depicting the three QD-based probes used in this study. The antiGFP-QD605 probe contains the rabbit <t>anti-GFP</t> IgG antibody combined with a secondary IgG antibody conjugated to QD605. The nanoGFP-QD605 probe contains an <t>anti-GFP</t> <t>nanobody</t> conjugated to QD605, while the nanoGFP-QD525 probe contains the anti-GFP nanobody conjugated to QD525. C , Negatively stained samples of both nanoGFP-QD probes were imaged at a magnification of 60,000× and a pixel size of 1.939 Å/px by TEM. Measured average diameters of both nanoGFP-QD probes ± SEM were 16.2 ± 0.4 nm (nanoGFP-QD525) and 20.4 ± 0.6 nm (nanoGFP-QD605; n ≥ 30). D , Example of drift estimated from multiple QD trajectories of the YFP-GluN1-1a subunit. E , The corresponding xy drift path. F , Example of raw QD trajectories (gray) and the drift-corrected QD trajectories (red) obtained by subtracting the drift path. G , Example images of fixed hippocampal neurons expressing the synaptic marker tdTomato-Homer1c (background pixels) and the YFP-GluN1-1a subunit labeled and tracked with the indicated QD-based probes (red). H , Scatter plots of all QD localizations; the red “+” indicates the mean value in both axes. I , Histograms showing the distribution of the distances between each QD localization shown in H ; the data were fitted with a Gaussian function, and the corresponding sigma (σ) values are indicated. J , Box plot summarizing the Gaussian fits performed on fixed QDs ( n = 20/group); one-way ANOVA F (2,57) = 121.84, p < 0.0001 followed by Bonferroni's multiple-comparisons test with p -values denoted in the figure. The average (mean ± SEM) localization errors were σ = 6.62 ± 0.25 nm (antiGFP-QD605), σ = 6.98 ± 0.32 nm (nanoGFP-QD605), and 15.34 ± 0.66 nm (nanoGFP-QD525).
    Bacterial Expression Vector Encoding Gfp Nanobody, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bacterial+expression+vector+encoding+gfp+nanobody/pOPINE+GFP+nanobody+(Plasmid+%2349172)/pmc10312064-66-1-10
    Average 93 stars, based on 42 article reviews
    bacterial expression vector encoding gfp nanobody - by Bioz Stars, 2026-09
    93/100 stars

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    1) Product Images from "Subunit-Dependent Surface Mobility and Localization of NMDA Receptors in Hippocampal Neurons Measured Using Nanobody Probes"

    Article Title: Subunit-Dependent Surface Mobility and Localization of NMDA Receptors in Hippocampal Neurons Measured Using Nanobody Probes

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.2014-22.2023

    Drift correction and localization error of QD probes in fixed hippocampal neurons expressing the YFP-tagged GluN1-1a subunit. A , Representative images of hippocampal neurons. Left, Neurons were stained by incubation in 20 n m MitoTracker Deep Red FM marker (catalog #M22426, Thermo Fisher Scientific) for 30 s in Neurobasal medium. Right, Neurons were infected with a lentivirus expressing the synaptic protein tdTomato-Homer1c. B , Schematic diagram depicting the three QD-based probes used in this study. The antiGFP-QD605 probe contains the rabbit anti-GFP IgG antibody combined with a secondary IgG antibody conjugated to QD605. The nanoGFP-QD605 probe contains an anti-GFP nanobody conjugated to QD605, while the nanoGFP-QD525 probe contains the anti-GFP nanobody conjugated to QD525. C , Negatively stained samples of both nanoGFP-QD probes were imaged at a magnification of 60,000× and a pixel size of 1.939 Å/px by TEM. Measured average diameters of both nanoGFP-QD probes ± SEM were 16.2 ± 0.4 nm (nanoGFP-QD525) and 20.4 ± 0.6 nm (nanoGFP-QD605; n ≥ 30). D , Example of drift estimated from multiple QD trajectories of the YFP-GluN1-1a subunit. E , The corresponding xy drift path. F , Example of raw QD trajectories (gray) and the drift-corrected QD trajectories (red) obtained by subtracting the drift path. G , Example images of fixed hippocampal neurons expressing the synaptic marker tdTomato-Homer1c (background pixels) and the YFP-GluN1-1a subunit labeled and tracked with the indicated QD-based probes (red). H , Scatter plots of all QD localizations; the red “+” indicates the mean value in both axes. I , Histograms showing the distribution of the distances between each QD localization shown in H ; the data were fitted with a Gaussian function, and the corresponding sigma (σ) values are indicated. J , Box plot summarizing the Gaussian fits performed on fixed QDs ( n = 20/group); one-way ANOVA F (2,57) = 121.84, p < 0.0001 followed by Bonferroni's multiple-comparisons test with p -values denoted in the figure. The average (mean ± SEM) localization errors were σ = 6.62 ± 0.25 nm (antiGFP-QD605), σ = 6.98 ± 0.32 nm (nanoGFP-QD605), and 15.34 ± 0.66 nm (nanoGFP-QD525).
    Figure Legend Snippet: Drift correction and localization error of QD probes in fixed hippocampal neurons expressing the YFP-tagged GluN1-1a subunit. A , Representative images of hippocampal neurons. Left, Neurons were stained by incubation in 20 n m MitoTracker Deep Red FM marker (catalog #M22426, Thermo Fisher Scientific) for 30 s in Neurobasal medium. Right, Neurons were infected with a lentivirus expressing the synaptic protein tdTomato-Homer1c. B , Schematic diagram depicting the three QD-based probes used in this study. The antiGFP-QD605 probe contains the rabbit anti-GFP IgG antibody combined with a secondary IgG antibody conjugated to QD605. The nanoGFP-QD605 probe contains an anti-GFP nanobody conjugated to QD605, while the nanoGFP-QD525 probe contains the anti-GFP nanobody conjugated to QD525. C , Negatively stained samples of both nanoGFP-QD probes were imaged at a magnification of 60,000× and a pixel size of 1.939 Å/px by TEM. Measured average diameters of both nanoGFP-QD probes ± SEM were 16.2 ± 0.4 nm (nanoGFP-QD525) and 20.4 ± 0.6 nm (nanoGFP-QD605; n ≥ 30). D , Example of drift estimated from multiple QD trajectories of the YFP-GluN1-1a subunit. E , The corresponding xy drift path. F , Example of raw QD trajectories (gray) and the drift-corrected QD trajectories (red) obtained by subtracting the drift path. G , Example images of fixed hippocampal neurons expressing the synaptic marker tdTomato-Homer1c (background pixels) and the YFP-GluN1-1a subunit labeled and tracked with the indicated QD-based probes (red). H , Scatter plots of all QD localizations; the red “+” indicates the mean value in both axes. I , Histograms showing the distribution of the distances between each QD localization shown in H ; the data were fitted with a Gaussian function, and the corresponding sigma (σ) values are indicated. J , Box plot summarizing the Gaussian fits performed on fixed QDs ( n = 20/group); one-way ANOVA F (2,57) = 121.84, p < 0.0001 followed by Bonferroni's multiple-comparisons test with p -values denoted in the figure. The average (mean ± SEM) localization errors were σ = 6.62 ± 0.25 nm (antiGFP-QD605), σ = 6.98 ± 0.32 nm (nanoGFP-QD605), and 15.34 ± 0.66 nm (nanoGFP-QD525).

    Techniques Used: Expressing, Staining, Incubation, Marker, Infection, Labeling

    Related Articles

    Expressing:

    Article Title: Subunit-Dependent Surface Mobility and Localization of NMDA Receptors in Hippocampal Neurons Measured Using Nanobody Probes
    Article Snippet: .. The bacterial expression vector encoding GFP nanobody (nanoGFP; catalog #49172, Addgene) was a gift from Brett Collins (The Institute for Molecular Bioscience, Queensland). .. NanoGFP including the C-terminal hexahistidine purification tag was expressed in Escherichia coli strain BL21 DE3 NiCo (New England Biolabs) in ZY autoinduction medium.

    Article Title: Subunit-Dependent Surface Mobility and Localization of NMDA Receptors in Hippocampal Neurons Measured Using Nanobody Probes
    Article Snippet: .. Preparation of GFP nanobody QD525 and GFP nanobody QD605 probes The bacterial expression vector encoding GFP nanobody (nanoGFP; catalog #49172, Addgene) was a gift from Brett Collins (The Institute for Molecular Bioscience, Queensland). .. NanoGFP including the C-terminal hexahistidine purification tag was expressed in Escherichia coli strain BL21 DE3 NiCo (New England Biolabs) in ZY autoinduction medium.

    Plasmid Preparation:

    Article Title: Subunit-Dependent Surface Mobility and Localization of NMDA Receptors in Hippocampal Neurons Measured Using Nanobody Probes
    Article Snippet: .. The bacterial expression vector encoding GFP nanobody (nanoGFP; catalog #49172, Addgene) was a gift from Brett Collins (The Institute for Molecular Bioscience, Queensland). .. NanoGFP including the C-terminal hexahistidine purification tag was expressed in Escherichia coli strain BL21 DE3 NiCo (New England Biolabs) in ZY autoinduction medium.

    Article Title: Subunit-Dependent Surface Mobility and Localization of NMDA Receptors in Hippocampal Neurons Measured Using Nanobody Probes
    Article Snippet: .. Preparation of GFP nanobody QD525 and GFP nanobody QD605 probes The bacterial expression vector encoding GFP nanobody (nanoGFP; catalog #49172, Addgene) was a gift from Brett Collins (The Institute for Molecular Bioscience, Queensland). .. NanoGFP including the C-terminal hexahistidine purification tag was expressed in Escherichia coli strain BL21 DE3 NiCo (New England Biolabs) in ZY autoinduction medium.



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    bacterial expression vector encoding gfp nanobody  (Addgene inc)
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    Addgene inc bacterial expression vector encoding gfp nanobody
    Drift correction and localization error of QD probes in fixed hippocampal neurons expressing the YFP-tagged GluN1-1a subunit. A , Representative images of hippocampal neurons. Left, Neurons were stained by incubation in 20 n m MitoTracker Deep Red FM marker (catalog #M22426, Thermo Fisher Scientific) for 30 s in Neurobasal medium. Right, Neurons were infected with a lentivirus expressing the synaptic protein tdTomato-Homer1c. B , Schematic diagram depicting the three QD-based probes used in this study. The antiGFP-QD605 probe contains the rabbit <t>anti-GFP</t> IgG antibody combined with a secondary IgG antibody conjugated to QD605. The nanoGFP-QD605 probe contains an <t>anti-GFP</t> <t>nanobody</t> conjugated to QD605, while the nanoGFP-QD525 probe contains the anti-GFP nanobody conjugated to QD525. C , Negatively stained samples of both nanoGFP-QD probes were imaged at a magnification of 60,000× and a pixel size of 1.939 Å/px by TEM. Measured average diameters of both nanoGFP-QD probes ± SEM were 16.2 ± 0.4 nm (nanoGFP-QD525) and 20.4 ± 0.6 nm (nanoGFP-QD605; n ≥ 30). D , Example of drift estimated from multiple QD trajectories of the YFP-GluN1-1a subunit. E , The corresponding xy drift path. F , Example of raw QD trajectories (gray) and the drift-corrected QD trajectories (red) obtained by subtracting the drift path. G , Example images of fixed hippocampal neurons expressing the synaptic marker tdTomato-Homer1c (background pixels) and the YFP-GluN1-1a subunit labeled and tracked with the indicated QD-based probes (red). H , Scatter plots of all QD localizations; the red “+” indicates the mean value in both axes. I , Histograms showing the distribution of the distances between each QD localization shown in H ; the data were fitted with a Gaussian function, and the corresponding sigma (σ) values are indicated. J , Box plot summarizing the Gaussian fits performed on fixed QDs ( n = 20/group); one-way ANOVA F (2,57) = 121.84, p < 0.0001 followed by Bonferroni's multiple-comparisons test with p -values denoted in the figure. The average (mean ± SEM) localization errors were σ = 6.62 ± 0.25 nm (antiGFP-QD605), σ = 6.98 ± 0.32 nm (nanoGFP-QD605), and 15.34 ± 0.66 nm (nanoGFP-QD525).
    Bacterial Expression Vector Encoding Gfp Nanobody, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bacterial+expression+vector+encoding+gfp+nanobody/pOPINE+GFP+nanobody+(Plasmid+%2349172)/pmc10312064-66-1-10
    Average 93 stars, based on 1 article reviews
    bacterial expression vector encoding gfp nanobody - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Drift correction and localization error of QD probes in fixed hippocampal neurons expressing the YFP-tagged GluN1-1a subunit. A , Representative images of hippocampal neurons. Left, Neurons were stained by incubation in 20 n m MitoTracker Deep Red FM marker (catalog #M22426, Thermo Fisher Scientific) for 30 s in Neurobasal medium. Right, Neurons were infected with a lentivirus expressing the synaptic protein tdTomato-Homer1c. B , Schematic diagram depicting the three QD-based probes used in this study. The antiGFP-QD605 probe contains the rabbit anti-GFP IgG antibody combined with a secondary IgG antibody conjugated to QD605. The nanoGFP-QD605 probe contains an anti-GFP nanobody conjugated to QD605, while the nanoGFP-QD525 probe contains the anti-GFP nanobody conjugated to QD525. C , Negatively stained samples of both nanoGFP-QD probes were imaged at a magnification of 60,000× and a pixel size of 1.939 Å/px by TEM. Measured average diameters of both nanoGFP-QD probes ± SEM were 16.2 ± 0.4 nm (nanoGFP-QD525) and 20.4 ± 0.6 nm (nanoGFP-QD605; n ≥ 30). D , Example of drift estimated from multiple QD trajectories of the YFP-GluN1-1a subunit. E , The corresponding xy drift path. F , Example of raw QD trajectories (gray) and the drift-corrected QD trajectories (red) obtained by subtracting the drift path. G , Example images of fixed hippocampal neurons expressing the synaptic marker tdTomato-Homer1c (background pixels) and the YFP-GluN1-1a subunit labeled and tracked with the indicated QD-based probes (red). H , Scatter plots of all QD localizations; the red “+” indicates the mean value in both axes. I , Histograms showing the distribution of the distances between each QD localization shown in H ; the data were fitted with a Gaussian function, and the corresponding sigma (σ) values are indicated. J , Box plot summarizing the Gaussian fits performed on fixed QDs ( n = 20/group); one-way ANOVA F (2,57) = 121.84, p < 0.0001 followed by Bonferroni's multiple-comparisons test with p -values denoted in the figure. The average (mean ± SEM) localization errors were σ = 6.62 ± 0.25 nm (antiGFP-QD605), σ = 6.98 ± 0.32 nm (nanoGFP-QD605), and 15.34 ± 0.66 nm (nanoGFP-QD525).

    Journal: The Journal of Neuroscience

    Article Title: Subunit-Dependent Surface Mobility and Localization of NMDA Receptors in Hippocampal Neurons Measured Using Nanobody Probes

    doi: 10.1523/JNEUROSCI.2014-22.2023

    Figure Lengend Snippet: Drift correction and localization error of QD probes in fixed hippocampal neurons expressing the YFP-tagged GluN1-1a subunit. A , Representative images of hippocampal neurons. Left, Neurons were stained by incubation in 20 n m MitoTracker Deep Red FM marker (catalog #M22426, Thermo Fisher Scientific) for 30 s in Neurobasal medium. Right, Neurons were infected with a lentivirus expressing the synaptic protein tdTomato-Homer1c. B , Schematic diagram depicting the three QD-based probes used in this study. The antiGFP-QD605 probe contains the rabbit anti-GFP IgG antibody combined with a secondary IgG antibody conjugated to QD605. The nanoGFP-QD605 probe contains an anti-GFP nanobody conjugated to QD605, while the nanoGFP-QD525 probe contains the anti-GFP nanobody conjugated to QD525. C , Negatively stained samples of both nanoGFP-QD probes were imaged at a magnification of 60,000× and a pixel size of 1.939 Å/px by TEM. Measured average diameters of both nanoGFP-QD probes ± SEM were 16.2 ± 0.4 nm (nanoGFP-QD525) and 20.4 ± 0.6 nm (nanoGFP-QD605; n ≥ 30). D , Example of drift estimated from multiple QD trajectories of the YFP-GluN1-1a subunit. E , The corresponding xy drift path. F , Example of raw QD trajectories (gray) and the drift-corrected QD trajectories (red) obtained by subtracting the drift path. G , Example images of fixed hippocampal neurons expressing the synaptic marker tdTomato-Homer1c (background pixels) and the YFP-GluN1-1a subunit labeled and tracked with the indicated QD-based probes (red). H , Scatter plots of all QD localizations; the red “+” indicates the mean value in both axes. I , Histograms showing the distribution of the distances between each QD localization shown in H ; the data were fitted with a Gaussian function, and the corresponding sigma (σ) values are indicated. J , Box plot summarizing the Gaussian fits performed on fixed QDs ( n = 20/group); one-way ANOVA F (2,57) = 121.84, p < 0.0001 followed by Bonferroni's multiple-comparisons test with p -values denoted in the figure. The average (mean ± SEM) localization errors were σ = 6.62 ± 0.25 nm (antiGFP-QD605), σ = 6.98 ± 0.32 nm (nanoGFP-QD605), and 15.34 ± 0.66 nm (nanoGFP-QD525).

    Article Snippet: The bacterial expression vector encoding GFP nanobody (nanoGFP; catalog #49172, Addgene) was a gift from Brett Collins (The Institute for Molecular Bioscience, Queensland).

    Techniques: Expressing, Staining, Incubation, Marker, Infection, Labeling